<SUP>12</SUP>C<SUP>6+</SUP>离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

赵卫平; 张红; #; 王燕玲; 李宁; 刘斌; 武振华; 谢漪; 郝冀方; 刘阳

doi:10.11804/NuclPhysRev.26.02.158

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

¹²C⁶⁺离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

赵卫平, 张红, #, 王燕玲, 李宁, 刘斌, 武振华, 谢漪, 郝冀方, 刘阳

文章导航 > 原子核物理评论 > 2009 > 26(2): 158-162

赵卫平, 张红, #, 王燕玲, 李宁, 刘斌, 武振华, 谢漪, 郝冀方, 刘阳. 12C6+离子预辐射对小鼠胸腺脾脏细胞周期进程的影响[J]. 原子核物理评论, 2009, 26(2): 158-162. doi: 10.11804/NuclPhysRev.26.02.158

引用本文:

赵卫平, 张红, #, 王燕玲, 李宁, 刘斌, 武振华, 谢漪, 郝冀方, 刘阳. ¹²C⁶⁺离子预辐射对小鼠胸腺脾脏细胞周期进程的影响[J]. 原子核物理评论, 2009, 26(2): 158-162. doi: 10.11804/NuclPhysRev.26.02.158

ZHAO Wei-ping, ZHANG Hong, #, WANG Yan-ling, XIE Yi, HAO Ji-fang, . Effect of Preirradiation with Low Dose 12C6+ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen[J]. Nuclear Physics Review, 2009, 26(2): 158-162. doi: 10.11804/NuclPhysRev.26.02.158

Citation:

ZHAO Wei-ping, ZHANG Hong, #, WANG Yan-ling, XIE Yi, HAO Ji-fang, . Effect of Preirradiation with Low Dose ¹²C⁶⁺ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen[J]. Nuclear Physics Review, 2009, 26(2): 158-162. doi: 10.11804/NuclPhysRev.26.02.158

¹²C⁶⁺离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

doi: 10.11804/NuclPhysRev.26.02.158

1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 中国科学院研究生院, 北京 100049; 3 甘肃省重离子束辐射生物医学重点实验室, 甘肃兰州 730000

详细信息

通讯作者: 张红

Effect of Preirradiation with Low Dose ¹²C⁶⁺ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 Key laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China

摘要: 以0， 0.05， 0.1， 0.25， 0.5 Gy ¹²C⁶⁺ 离子全身预辐照昆明小鼠，间隔4 h后再对小鼠进行4 Gy全身辐射。辐照后12 h用流式细胞仪检测小鼠胸腺脾脏细胞在各细胞周期时相的百分率，同时用单细胞电泳检测受辐射小鼠胸腺脾脏细胞DNA损伤程度。结果显示，相对于大剂量预照射组，各低剂量预照射组胸腺细胞S期细胞百分率显著减少；脾脏细胞G0/G1期细胞百分率明显减少；同时胸腺脾脏细胞的拖尾率及拖尾长度明显减少，以0.1 Gy预辐照效果最为明显。这些结果表明，低剂量预辐射处理可以减弱胸腺细胞的S期阻滞及脾脏细胞的G1期阻滞，并明显减轻胸腺脾脏细胞的DNA损伤程度。
- 低剂量碳离子 /
- 预辐射 /
- 细胞周期 /
- DNA损伤
Abstract: To investigate the effect of low dose ¹²C⁶⁺ preirradiation on the cell cycle progression and DNA damage in mouse thymus and spleen， KunMing strain mice were wholebody irradiated with 0， 0.05， 0.1， 0.25 or 0.5 Gy of 12C6+ ions as the preexposure dose， and were then irradiated with 4 Gy of 12C6+ ions as a challenging dose at 4 h after perexposure. At 12h after irradiation， cell cycles of thymus and spleen were analyzed by FACS， and the frequencies of cells with tail moment and the tail lengths were determined by the single cell gel electrophoresis. The results showed that compared with highdose exposure group， the percentages of S phase cells on thymus and the percentages of G0/G1 phase cells on spleen significantly decreased via pre exposure to lowdose ¹²C⁶⁺ ions. Moreover， the lowdose preexposure significantly reduced length of the tails and the number of cells with tail， especially at the 0.1 Gy preexposure. The data suggested that preexposure to lowdose heavy ion can alleviate S phase arrest in thymus cells and the G1 phase arrest in spleen cell， and the degree of DNA damage.
- low dose 12C6+ ion /
- preirradiation /
- cell cycle /
- DNA damage

点击查看大图

计量

文章访问数: 2954
HTML全文浏览量: 92
PDF下载量: 717
被引次数: 0

¹²C⁶⁺离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

doi: 10.11804/NuclPhysRev.26.02.158

1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 中国科学院研究生院, 北京 100049; 3 甘肃省重离子束辐射生物医学重点实验室, 甘肃兰州 730000

通讯作者: 张红

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2009-06-20

关键词:

低剂量碳离子 /

预辐射 /

细胞周期 /

DNA损伤

摘要: 以0， 0.05， 0.1， 0.25， 0.5 Gy ¹²C⁶⁺ 离子全身预辐照昆明小鼠，间隔4 h后再对小鼠进行4 Gy全身辐射。辐照后12 h用流式细胞仪检测小鼠胸腺脾脏细胞在各细胞周期时相的百分率，同时用单细胞电泳检测受辐射小鼠胸腺脾脏细胞DNA损伤程度。结果显示，相对于大剂量预照射组，各低剂量预照射组胸腺细胞S期细胞百分率显著减少；脾脏细胞G0/G1期细胞百分率明显减少；同时胸腺脾脏细胞的拖尾率及拖尾长度明显减少，以0.1 Gy预辐照效果最为明显。这些结果表明，低剂量预辐射处理可以减弱胸腺细胞的S期阻滞及脾脏细胞的G1期阻滞，并明显减轻胸腺脾脏细胞的DNA损伤程度。

English Abstract

Effect of Preirradiation with Low Dose ¹²C⁶⁺ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 Key laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2009-06-20

Keywords:

Abstract: To investigate the effect of low dose ¹²C⁶⁺ preirradiation on the cell cycle progression and DNA damage in mouse thymus and spleen， KunMing strain mice were wholebody irradiated with 0， 0.05， 0.1， 0.25 or 0.5 Gy of 12C6+ ions as the preexposure dose， and were then irradiated with 4 Gy of 12C6+ ions as a challenging dose at 4 h after perexposure. At 12h after irradiation， cell cycles of thymus and spleen were analyzed by FACS， and the frequencies of cells with tail moment and the tail lengths were determined by the single cell gel electrophoresis. The results showed that compared with highdose exposure group， the percentages of S phase cells on thymus and the percentages of G0/G1 phase cells on spleen significantly decreased via pre exposure to lowdose ¹²C⁶⁺ ions. Moreover， the lowdose preexposure significantly reduced length of the tails and the number of cells with tail， especially at the 0.1 Gy preexposure. The data suggested that preexposure to lowdose heavy ion can alleviate S phase arrest in thymus cells and the G1 phase arrest in spleen cell， and the degree of DNA damage.

引用本文:

Citation:

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

留言板

12C6+离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

doi: 10.11804/NuclPhysRev.26.02.158

通讯作者: 张红

Effect of Preirradiation with Low Dose 12C6+ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen

计量

出版历程

12C6+离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

doi: 10.11804/NuclPhysRev.26.02.158

1 中国科学院近代物理研究所, 甘肃 兰州 730000; 2 中国科学院研究生院, 北京 100049; 3 甘肃省重离子束辐射生物医学重点实验室, 甘肃 兰州 730000

通讯作者: 张红

English Abstract

Effect of Preirradiation with Low Dose 12C6+ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 Key laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China

全文HTML

目录

¹²C⁶⁺离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

Effect of Preirradiation with Low Dose ¹²C⁶⁺ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen

¹²C⁶⁺离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 中国科学院研究生院, 北京 100049; 3 甘肃省重离子束辐射生物医学重点实验室, 甘肃兰州 730000

Effect of Preirradiation with Low Dose ¹²C⁶⁺ Ions on Cell Cycle Progression and DNA Damage in Mouse Thymus and Spleen