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摘要: 在具有高效护水功能石蜡油的保护下进行了人宫颈癌细胞(HeLa细胞)的低能离子注入及真空处理, 用荧光定量PCR的方法分别研究了真空和30 keV N+的不同注入剂量下, 细胞中p53基因和c-fos基因的mRNA表达变化。 结果表明, 上述两个基因的表达与真空及低能离子注入之间存在着剂量关系。Abstract: The relationship between gene expression and lowenergy ion implantation has been studied. Because vacuum is prerequisite in lowenergy ion implantation, mineral oil was used to protect cells from water evaporating. In this study, HeLa cells were implanted by lowenergy ions (30 keV N+) at different doses, and p53 gene and c fos gene were studied with realtime quantitative PCR. The result shows that gene expression changed obviously when cells were acted as vacuum control to the sample implanted 5×1014 ions/cm2.
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Key words:
- vacuum /
- lowenergy ion /
- HeLa cell /
- gene expression /
- realtime quantitative PCR
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