关键词: 
辐射诱导的远程表观遗传效应 /
甲基化 /
AtDML3 基因

摘要: 由于低能离子较低的组织穿透能力， 其诱变机理一直是研究者争论的问题。 近年来， 本研究组的一系列研究工作已经证明在植物中存在辐射远程（诱变）效应， 从一个新的角度解释了低能离子的诱变机理， 然而依然无法解释低能离子辐照中的许多独特生物现象， 而这些现象均具有明显的表观遗传学特性。 以表观遗传学最具标志性特征的胞嘧啶甲基化为研究对象， 以α粒子拟南芥菜根辐照实验体系作为研究平台， 检测了远程组织(器官)甲基化相关基因 AtDML3 的表达及特定基因片段的甲基化水平。 研究证实， 在植物个体水平辐射可以诱导远程表观遗传的变化， 为进一步探索低能离子的诱变机理提供了新的思路。 Along the way, the mutagenic mechanism of lowenergy ions irradiation is a debatable issue. Recently, the existence of radiation induced longrange (mutagenic) effects in vivo in plants has been performed in a series of studies of our group, which account for the mutagenesis of lowenergy ions irradiation in a new perspective. However, numerous distinct biology phenomena remain to be addressed, which bear obvious characteristics to epigenetic. In the present study, using the expression of methylationrelated AtDML3 gene and methylation level of specific gene segments as end points, the methylation of cytosine, the most important feature of epigenetic, was investigated. It was shown that, in A. thaliana, rootloca lized αirradiation could induce epigenetic changes in aerial parts which avoided the direct irradiation. The radiation induced longrange epigenetic changes were confirmed in this study, which supplied innovative ideas for the further investigation of the mutagenetic mechanism of lowenergy ions irradiation.