关键词: 
长链非编码RNA /
电离辐射 /
RNA测序 /
实时荧光定量PCR

摘要: 长链非编码RNA(lncRNA) 是长度超过200 nt、不具有蛋白编码功能的转录本，它们在真核生物中发挥基因调控等重要作用。迄今为止，还没有检索到有关参与电离辐射应答的lncRNA 研究报道。据推测，细胞暴露于电离辐射之后有些lncRNA 的转录水平将发生变化。因此，采用2 Gy 的X射线辐照HeLa 细胞，辐照后4 h 提取总RNA 进行全序列测序。最终发现1 028 种lncRNA 的转录水平发生了改变，其中包括381 种新lncRNA。从中随机挑选24 种lncRNA，用实时荧光定量PCR法验证了2 Gy 的X射线或80 keV/m碳离子束照射后的表达变化，发现其变化趋势和测序结果一致。此研究为电离辐射应答相关lncRNA 的功能研究奠定了基础。

Long non-coding RNAs (lncRNAs) are a class of non-coding transcripts longer than 200 nt and potentially play important roles in eukaryotic gene regulation. However, the quantity, characteristics and expression pattern of lncRNAs responding to ionizing radiation remain largely unknown. We speculate that some lncRNAs participate in the induction of radiobiological effects and ionizing radiation changes their expression levels. In the present study, total RNAs of HeLa cells were isolated 4 h after exposure to 2 Gy of X-rays, and sequenced by lncRNA-seq technology. We identified 647 kinds of known lncRNAs and 381 kinds of novel non-coding sequences responding to ionizing radiation. Subsequently, we verified the expression of 24 lncRNAs after exposure to 2 Gy
X-rays or 80 keV/m carbon ions by using qRT-PCR.