和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

蔺兴遥; 刘炳涛; 马晓辉; 张扬; 陈卫强; 金晓东

doi:10.11804/NuclPhysRev.36.01.104

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和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

蔺兴遥, 刘炳涛, 马晓辉, 张扬, 陈卫强, 金晓东

文章导航 > 原子核物理评论 > 2019 > 36(1): 104-110

蔺兴遥, 刘炳涛, 马晓辉, 张扬, 陈卫强, 金晓东. 和厚朴酚对非小细胞肺癌细胞的辐射增敏效应[J]. 原子核物理评论, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104

引用本文:

MING Xingyao, LIU Bingtao, MA Xiaohui, ZHANG Yang, CHEN Weiqiang, JIN Xiaodong. Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells[J]. Nuclear Physics Review, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104

Citation:

MING Xingyao, LIU Bingtao, MA Xiaohui, ZHANG Yang, CHEN Weiqiang, JIN Xiaodong. Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells[J]. Nuclear Physics Review, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104

和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

doi: 10.11804/NuclPhysRev.36.01.104

蔺兴遥^1,2,
刘炳涛^3,4,
马晓辉^1,2,
张扬^1,2,
陈卫强^3,4,
金晓东^3,4,

1.
甘肃中医药大学科研实验中心, 兰州 730000;
2.
敦煌医学与转化教育部重点实验室, 兰州 730000;
3.
中国科学院近代物理研究所, 兰州 730000;
4.
中国科学院大学核科学与技术学院, 北京 100049

基金项目: 国家自然科学基金资助项目（U1730133）；甘肃省自然科学基金资助项目（17JRSRA310）

详细信息

作者简介:
蔺兴遥(1975-),男,甘肃靖远人,副教授,硕士,从事中药药理与毒理研究;Email:878104134@qq.com

通讯作者: 金晓东,E-mail:jinxd@imocas.ac.cn

中图分类号: R730.55;R96

Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells

1.
Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;
2.
Key Laboratory of Dunhuang Medicine Ministry of Education, Lanzhou 730000, China;
3.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
4.
School of Nuclear Science and Technology, University of Chinese Academy of Sciences, Beijing 100049, China

Funds: National Natural Science Foundation of China (U1730133); Natural Science Foundation of Gansu Province (17JRSRA310)

摘要: 研究了和厚朴酚（HNK）对非小细胞肺癌（NSCLC）细胞系A549和H1299对低线性能量转移（LET） X射线和高LET碳离子的辐射增敏效应。首先用CCK-8检测了HNK对A549和H1299细胞的生长抑制情况，发现20 μmol/L的HNK处理对细胞的生长抑制作用较弱。用该浓度HNK预处理细胞2 h后给予不同剂量X射线或碳离子的照射，克隆存活法检测细胞的辐射敏感性，Annexin-PI双染法检测细胞凋亡，γH2AX焦点法检测DNA的双链断裂（DSB）损伤。实验结果显示：与X射线相比，NSCLC细胞对碳离子更敏感，HNK预处理仅对碳离子照射有辐射增敏作用；与碳离子单独照射相比，HNK预处理联合碳离子照射诱导了更明显的细胞凋亡；在照射后24 h，HNK预处理联合碳离子照射引起的细胞γH2AX焦点阳性率维持在较高水平，而X射线照射没有这些效应。实验结果表明，HNK预处理抑制了NSCLC细胞DNA的DSB修复，诱导了细胞凋亡的发生，从而提高了细胞对碳离子的辐射敏感性。

The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.
- 和厚朴酚 /
- 非小细胞肺癌细胞 /
- X射线 /
- 碳离子 /
- 辐射增敏
Abstract: The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.
- Honokiol /
- non-small cell lung carcinoma cells /
- X-rays /
- carbon ions /
- radiosensitization

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[2]	AUPERIN A, C LE PECHOUX, ROLLAND E, et al. J Clin Oncol, 2010, 28:2181.
[3]	OLEINICK N L, BISWAS T, PATEL R, et al. Radiother Oncol 2016, 121:335.
[4]	KAMADA T, TSUJⅡ H, BLAKELY E A, et al. Lancet Oncol, 2015, 16:e93.
[5]	PAN J, ZHANG Q, LI K, et al. Cancer Prev Res (Phila), 2013, 6:530.
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[7]	HE Z, SUBRAMANIAM D, RAMALINGAM S, et al. Am J Physiol Gastrointest Liver Physiol, 2011, 301:G929.
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和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

doi: 10.11804/NuclPhysRev.36.01.104

1. 甘肃中医药大学科研实验中心, 兰州 730000;

2. 敦煌医学与转化教育部重点实验室, 兰州 730000;

3. 中国科学院近代物理研究所, 兰州 730000;

4. 中国科学院大学核科学与技术学院, 北京 100049

基金项目: 国家自然科学基金资助项目（U1730133）；甘肃省自然科学基金资助项目（17JRSRA310）

作者简介:
蔺兴遥(1975-),男,甘肃靖远人,副教授,硕士,从事中药药理与毒理研究;Email:878104134@qq.com

通讯作者: 金晓东,E-mail:jinxd@imocas.ac.cn

收稿日期: 2018-12-14
修回日期: 2019-02-02
刊出日期: 2019-03-20

中图分类号: R730.55;R96

关键词:

和厚朴酚 /

非小细胞肺癌细胞 /

X射线 /

碳离子 /

辐射增敏

摘要: 研究了和厚朴酚（HNK）对非小细胞肺癌（NSCLC）细胞系A549和H1299对低线性能量转移（LET） X射线和高LET碳离子的辐射增敏效应。首先用CCK-8检测了HNK对A549和H1299细胞的生长抑制情况，发现20 μmol/L的HNK处理对细胞的生长抑制作用较弱。用该浓度HNK预处理细胞2 h后给予不同剂量X射线或碳离子的照射，克隆存活法检测细胞的辐射敏感性，Annexin-PI双染法检测细胞凋亡，γH2AX焦点法检测DNA的双链断裂（DSB）损伤。实验结果显示：与X射线相比，NSCLC细胞对碳离子更敏感，HNK预处理仅对碳离子照射有辐射增敏作用；与碳离子单独照射相比，HNK预处理联合碳离子照射诱导了更明显的细胞凋亡；在照射后24 h，HNK预处理联合碳离子照射引起的细胞γH2AX焦点阳性率维持在较高水平，而X射线照射没有这些效应。实验结果表明，HNK预处理抑制了NSCLC细胞DNA的DSB修复，诱导了细胞凋亡的发生，从而提高了细胞对碳离子的辐射敏感性。

The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.

English Abstract

Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells

1. Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;

2. Key Laboratory of Dunhuang Medicine Ministry of Education, Lanzhou 730000, China;

3. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;

4. School of Nuclear Science and Technology, University of Chinese Academy of Sciences, Beijing 100049, China

Funds: National Natural Science Foundation of China (U1730133); Natural Science Foundation of Gansu Province (17JRSRA310)

Received Date: 2018-12-14
Rev Recd Date: 2019-02-02
Publish Date: 2019-03-20

Keywords:

Abstract: The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.

引用本文: