Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells

DUAN Xin; ZHANG Hong; QIU Rong; GAO Qing-xiang; MIN Feng-ling; HAO Ji-fang; LIU Bing; ZHOU Qing-ming; LI Xiao-da; WANG Yan-ling; ZHOU Guang-ming

doi:10.11804/NuclPhysRev.24.01.055

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Article Navigation > Nuclear Physics Review > 2007 > 24(1): 55-58

DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. doi: 10.11804/NuclPhysRev.24.01.055

Citation:

DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. doi: 10.11804/NuclPhysRev.24.01.055

Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells

doi: 10.11804/NuclPhysRev.24.01.055

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 School of Life Science, Lanzhou University, Lanzhou, 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2007-03-20

Abstract

The effect of ¹²C⁶⁺ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of ¹²C⁶⁺ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that ¹²C⁶⁺ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.
- ¹²C^6＋heavyion beam,
- AdCMVGFP,
- transfection efficiency,
- murine melanoma
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells

doi: 10.11804/NuclPhysRev.24.01.055

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 School of Life Science, Lanzhou University, Lanzhou, 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2007-03-20

Key words:

Abstract: The effect of ¹²C⁶⁺ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of ¹²C⁶⁺ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that ¹²C⁶⁺ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.

Citation:

DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. doi: 10.11804/NuclPhysRev.24.01.055