High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray

ZHANG Hong; LIU  Bing; GAO  Qing-xiang; XIE Yi; HAO  Ji-fang; DUAN Xin; ZHOU  Qing-ming; WANG Yan-ling

doi:10.11804/NuclPhysRev.24.02.112

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Article Navigation > Nuclear Physics Review > 2007 > 24(2): 112-116

ZHANG Hong, LIU Bing, GAO Qing-xiang, XIE Yi, HAO Ji-fang, DUAN Xin, ZHOU Qing-ming, WANG Yan-ling. High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray[J]. Nuclear Physics Review, 2007, 24(2): 112-116. doi: 10.11804/NuclPhysRev.24.02.112

Citation:

ZHANG Hong, LIU Bing, GAO Qing-xiang, XIE Yi, HAO Ji-fang, DUAN Xin, ZHOU Qing-ming, WANG Yan-ling. High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray[J]. Nuclear Physics Review, 2007, 24(2): 112-116. doi: 10.11804/NuclPhysRev.24.02.112

High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray

doi: 10.11804/NuclPhysRev.24.02.112

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Lanzhou Command Center for Disease Prevention and Control, Lanzhou 730020 China; 3 School of Life Sciences, Lanzhou University, Lanzhou 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2007-06-20

Abstract

To determine whether lowdose preirradiation could enhance adenovirusmediated p53 transfer and expression in human prostate adenocarcinoma， the PC3 cells were preexposed to γ rays， and then infected with replicationdeficient adenovirus recombinant vectors， containing human wildtype p53 (AdCMVp53) or green fluorescent protein gene (AdCMVGFP) respectively (γray irradiation + AdCMVp53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMVGFP infection groups were 7%—39% more than those in AdCMVGFP infection groups. The p53 levels in the γ irradiation + AdCMVp53 infection groups were 18.5%—35.4% more than those in AdCMVp53 infection groups (p<0.05)，suggesting that lowdose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC3 cells. The survival fractions for the γ irradiation + AdCMVp53 infection groups were 25%—64%， 22%—65% less than those for AdCMVp53 infection， or γirradiation groups， respectively (p<0.05).
- p53-gene,
- irradiation,
- adenovirus vector,
- prostate adenocarcinoma,
- radiosensitivity
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray

doi: 10.11804/NuclPhysRev.24.02.112

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Lanzhou Command Center for Disease Prevention and Control, Lanzhou 730020 China; 3 School of Life Sciences, Lanzhou University, Lanzhou 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2007-06-20

Key words:

Abstract: To determine whether lowdose preirradiation could enhance adenovirusmediated p53 transfer and expression in human prostate adenocarcinoma， the PC3 cells were preexposed to γ rays， and then infected with replicationdeficient adenovirus recombinant vectors， containing human wildtype p53 (AdCMVp53) or green fluorescent protein gene (AdCMVGFP) respectively (γray irradiation + AdCMVp53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMVGFP infection groups were 7%—39% more than those in AdCMVGFP infection groups. The p53 levels in the γ irradiation + AdCMVp53 infection groups were 18.5%—35.4% more than those in AdCMVp53 infection groups (p<0.05)，suggesting that lowdose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC3 cells. The survival fractions for the γ irradiation + AdCMVp53 infection groups were 25%—64%， 22%—65% less than those for AdCMVp53 infection， or γirradiation groups， respectively (p<0.05).

Citation:

ZHANG Hong, LIU Bing, GAO Qing-xiang, XIE Yi, HAO Ji-fang, DUAN Xin, ZHOU Qing-ming, WANG Yan-ling. High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray[J]. Nuclear Physics Review, 2007, 24(2): 112-116. doi: 10.11804/NuclPhysRev.24.02.112