Influence of Heavy Ion Irradiation on Proteome Profile of Human Hepatoma HepG2 Cells

WU Qing-feng; LI Qiang; #; JIN Xiao-dong

doi:10.11804/NuclPhysRev.25.02.148

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WU Qing-feng, LI Qiang, #, JIN Xiao-dong. Influence of Heavy Ion Irradiation on Proteome Profile of Human Hepatoma HepG2 Cells[J]. Nuclear Physics Review, 2008, 25(2): 148-153. doi: 10.11804/NuclPhysRev.25.02.148

Citation:

WU Qing-feng, LI Qiang, #, JIN Xiao-dong. Influence of Heavy Ion Irradiation on Proteome Profile of Human Hepatoma HepG2 Cells[J]. Nuclear Physics Review, 2008, 25(2): 148-153. doi: 10.11804/NuclPhysRev.25.02.148

Influence of Heavy Ion Irradiation on Proteome Profile of Human Hepatoma HepG2 Cells

doi: 10.11804/NuclPhysRev.25.02.148

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-06-20

Abstract

To gain a better understanding of the molecular basis of the biological effects induced by heavy ion irradiation， total proteins extracted from human hepatoma HepG2 cells that unirradiated and irradiated with an intermediateenergy carbon ion beam at 1 Gy were separated by twodimensional gel electrophoresis (2DE) with immobilized pH gradients (IPGs). The 2DE profiles were analyzed with the ImageMaster 2D software. More than 500 proteins spots were reproducibly detected on each gel and 17 were differentially expressed for the unirradiated and irradiated cells. 1 protein was expressed only in the unirradiated cells. 8 proteins were downregulated and 8 proteins were upregulated after the carbon ion irradiation. In this study， reproducible 2DE profiles were established and some differential expressed proteins were detected. These basic data are very useful for the further study of heavyion biological effect.
- HepG2 cell,
- heavy ion irradiation,
- proteome,
- twodimensional gel electrophoresis
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Influence of Heavy Ion Irradiation on Proteome Profile of Human Hepatoma HepG2 Cells

doi: 10.11804/NuclPhysRev.25.02.148

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-06-20

Key words:

Abstract: To gain a better understanding of the molecular basis of the biological effects induced by heavy ion irradiation， total proteins extracted from human hepatoma HepG2 cells that unirradiated and irradiated with an intermediateenergy carbon ion beam at 1 Gy were separated by twodimensional gel electrophoresis (2DE) with immobilized pH gradients (IPGs). The 2DE profiles were analyzed with the ImageMaster 2D software. More than 500 proteins spots were reproducibly detected on each gel and 17 were differentially expressed for the unirradiated and irradiated cells. 1 protein was expressed only in the unirradiated cells. 8 proteins were downregulated and 8 proteins were upregulated after the carbon ion irradiation. In this study， reproducible 2DE profiles were established and some differential expressed proteins were detected. These basic data are very useful for the further study of heavyion biological effect.

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