ZHANG  Feng-qiu; FANG  Hua-sheng; YA  Hui-yuan; QIN  Guang-yong

doi:10.11804/NuclPhysRev.25.04.385

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Volume 25 Issue 4

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Article Navigation > Nuclear Physics Review > 2008 > 25(4): 385-390

doi: 10.11804/NuclPhysRev.25.04.385

School of Physical Science & Technology, Zhengzhou University, Zhengzhou 450052, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-12-20

Abstract

The relationship between gene expression and lowenergy ion implantation has been studied. Because vacuum is prerequisite in lowenergy ion implantation， mineral oil was used to protect cells from water evaporating. In this study， HeLa cells were implanted by lowenergy ions (30 keV N⁺) at different doses, and p53 gene and c fos gene were studied with realtime quantitative PCR. The result shows that gene expression changed obviously when cells were acted as vacuum control to the sample implanted 5×1014 ions/cm².
- vacuum,
- lowenergy ion,
- HeLa cell,
- gene expression,
- realtime quantitative PCR
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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真空、低能离子注入对人宫颈癌（HeLa）细胞中 p53及c-fos mRNA的表达影响

doi: 10.11804/NuclPhysRev.25.04.385

School of Physical Science & Technology, Zhengzhou University, Zhengzhou 450052, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-12-20

Key words:

Abstract: The relationship between gene expression and lowenergy ion implantation has been studied. Because vacuum is prerequisite in lowenergy ion implantation， mineral oil was used to protect cells from water evaporating. In this study， HeLa cells were implanted by lowenergy ions (30 keV N⁺) at different doses, and p53 gene and c fos gene were studied with realtime quantitative PCR. The result shows that gene expression changed obviously when cells were acted as vacuum control to the sample implanted 5×1014 ions/cm².