Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation

DU  Bin-bin; LIU  Qing; ZHANG  Wei-sheng; WANG  Xiao-yan; JIA  Pen-fei; WANG  Xiao-hu; LI  Sha; YANG  Xiong-fei; ZHANG  Hong; Guo Tian-kang; #

doi:10.11804/NuclPhysRev.25.04.391

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Volume 25 Issue 4

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Article Navigation > Nuclear Physics Review > 2008 > 25(4): 391-396

DU Bin-bin, LIU Qing, ZHANG Wei-sheng, WANG Xiao-yan, JIA Pen-fei, WANG Xiao-hu, LI Sha, YANG Xiong-fei, ZHANG Hong, Guo Tian-kang, #. Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation[J]. Nuclear Physics Review, 2008, 25(4): 391-396. doi: 10.11804/NuclPhysRev.25.04.391

Citation:

DU Bin-bin, LIU Qing, ZHANG Wei-sheng, WANG Xiao-yan, JIA Pen-fei, WANG Xiao-hu, LI Sha, YANG Xiong-fei, ZHANG Hong, Guo Tian-kang, #. Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation[J]. Nuclear Physics Review, 2008, 25(4): 391-396. doi: 10.11804/NuclPhysRev.25.04.391

Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation

doi: 10.11804/NuclPhysRev.25.04.391

1 Clinical Medical College, Lanzhou University, Lanzhou 730000, China;2 Institute Of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;3 People’s Hospital of Gansu Province, Lanzhou 730000, China;4 School of Life Science, Lanzhou University, Lanzhou 730000, China;5 Gansu Tumor Hospital, Lanzhou 730050, China;6 General Hospital of Lanzhou Military Area, Lanzhou 730050, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-12-20

Abstract

To investigate the role of NADPH oxidase in HeLa cell lesion induced by Xray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laserscanning microscopy was employed to detect the colocalization of two subunit of NADPH oxidase, p47^phox and gp91^phox in the cell. Western blotting was used to detect the expression of gp91^phox before and after Xray irradiation. After Xray irradiation， intracellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47^phox moved to membrane and colocalizated with gp91^phox after
irradiation. Moreover, the results also show that gp91^phox increased sharply after 12 Gy Xray irradiation. Therefore， NADPH oxidasemediated production of ROS plays an important role in HeLa cell lesion induced by Xray.
- NADPH oxidase,
- reactive oxygen species,
- Xray,
- HeLa cell
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation

doi: 10.11804/NuclPhysRev.25.04.391

1 Clinical Medical College, Lanzhou University, Lanzhou 730000, China;2 Institute Of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;3 People’s Hospital of Gansu Province, Lanzhou 730000, China;4 School of Life Science, Lanzhou University, Lanzhou 730000, China;5 Gansu Tumor Hospital, Lanzhou 730050, China;6 General Hospital of Lanzhou Military Area, Lanzhou 730050, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-12-20

Key words:

Abstract: To investigate the role of NADPH oxidase in HeLa cell lesion induced by Xray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laserscanning microscopy was employed to detect the colocalization of two subunit of NADPH oxidase, p47^phox and gp91^phox in the cell. Western blotting was used to detect the expression of gp91^phox before and after Xray irradiation. After Xray irradiation， intracellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47^phox moved to membrane and colocalizated with gp91^phox after
irradiation. Moreover, the results also show that gp91^phox increased sharply after 12 Gy Xray irradiation. Therefore， NADPH oxidasemediated production of ROS plays an important role in HeLa cell lesion induced by Xray.

Citation:

DU Bin-bin, LIU Qing, ZHANG Wei-sheng, WANG Xiao-yan, JIA Pen-fei, WANG Xiao-hu, LI Sha, YANG Xiong-fei, ZHANG Hong, Guo Tian-kang, #. Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation[J]. Nuclear Physics Review, 2008, 25(4): 391-396. doi: 10.11804/NuclPhysRev.25.04.391