Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes

Key words: 
• X-ray /
• radiation /
• Eruca sativa Mill /
• hairy root

Abstract: To investigate the effects of X-ray irradiation to the hairy root induction of Eruca sativa Mill， and to provide basic experimental data for concerted reaction of irradiation to the Genetic transformation technology. The aseptic cotyledons of Eruca sativa Mill were jointly treated with the Xray at dose of 5， 10， 15， 20 Gy respectively and the different infected time， then the influence of explant age， preculturing time， dose of Xray and infected time were studied. Furthermore， the hairy roots were testified in molecular level by the polymerase chain reaction（PCR）. The irradiation of Xray at dose of 15 Gy can improve the frequency of Eruca sativa Mill hairy root induction in a dosedependent manner. Moreover， the transformation frequency in preinfection plus irradiation group is higher than that in preirradiation plus infection group at the same irradiation dose. In addition， the PCR analysis also demonstrated that rolB gene of TDNA from Ri plasmid had been integrated into the genome of the transformed roots. The irradiation of Xray has some positive effects on the hairy root induction of Eruca sativa Mill， and the optimal revulsive dose is 15 Gy or a little bit more.