Study on Yeast Mutant with High Alcohol Yield Fermented in Sweet Sorghum Juice Using Carbon Ion Irradiation

YAN Ya-ping; WANG Ju-fang; #; LU Dong; DONG Xi-cun; GAO Feng; MA Liang; LI Wen-jian

doi:10.11804/NuclPhysRev.26.03.269

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Article Navigation > Nuclear Physics Review > 2009 > 26(3): 269-273

YAN Ya-ping, WANG Ju-fang, #, LU Dong, DONG Xi-cun, GAO Feng, MA Liang, LI Wen-jian. Study on Yeast Mutant with High Alcohol Yield Fermented in Sweet Sorghum Juice Using Carbon Ion Irradiation[J]. Nuclear Physics Review, 2009, 26(3): 269-273. doi: 10.11804/NuclPhysRev.26.03.269

Citation:

YAN Ya-ping, WANG Ju-fang, #, LU Dong, DONG Xi-cun, GAO Feng, MA Liang, LI Wen-jian. Study on Yeast Mutant with High Alcohol Yield Fermented in Sweet Sorghum Juice Using Carbon Ion Irradiation[J]. Nuclear Physics Review, 2009, 26(3): 269-273. doi: 10.11804/NuclPhysRev.26.03.269

Study on Yeast Mutant with High Alcohol Yield Fermented in Sweet Sorghum Juice Using Carbon Ion Irradiation

doi: 10.11804/NuclPhysRev.26.03.269

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2009-09-20

Abstract

Five mutants with high ability of producing alcohol were selected out by using TTC as an indicator after irradiation of the alcohol yeast with 100 MeV/u carbon ions. The fermentation experiment in sweet sorghum juice showed that the alcohol production ability of mutant T4 strain increased 18.6% compared to the control strain. The residual sugar content in the juice was decreased too. After that, the optimum fermentation conditions of the T4 strain in sweet sorghum juice were investigated. The results showed that the optimum temperature and pH value for fermentation were 30 ℃ and 4.5, respectively. The verification experiment was fermented in a 10 l bioreactor and the obtained data indicated that the fermentative rate and the ability of producing alcohol in T4 strain was higher than that in the control strain under the same fermentation condition.
- heavy ion beam,
- alcohol yeast,
- sweet sorghum juice,
- fermentation
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Study on Yeast Mutant with High Alcohol Yield Fermented in Sweet Sorghum Juice Using Carbon Ion Irradiation

doi: 10.11804/NuclPhysRev.26.03.269

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2009-09-20

Key words:

Abstract: Five mutants with high ability of producing alcohol were selected out by using TTC as an indicator after irradiation of the alcohol yeast with 100 MeV/u carbon ions. The fermentation experiment in sweet sorghum juice showed that the alcohol production ability of mutant T4 strain increased 18.6% compared to the control strain. The residual sugar content in the juice was decreased too. After that, the optimum fermentation conditions of the T4 strain in sweet sorghum juice were investigated. The results showed that the optimum temperature and pH value for fermentation were 30 ℃ and 4.5, respectively. The verification experiment was fermented in a 10 l bioreactor and the obtained data indicated that the fermentative rate and the ability of producing alcohol in T4 strain was higher than that in the control strain under the same fermentation condition.

Citation:

YAN Ya-ping, WANG Ju-fang, #, LU Dong, DONG Xi-cun, GAO Feng, MA Liang, LI Wen-jian. Study on Yeast Mutant with High Alcohol Yield Fermented in Sweet Sorghum Juice Using Carbon Ion Irradiation[J]. Nuclear Physics Review, 2009, 26(3): 269-273. doi: 10.11804/NuclPhysRev.26.03.269