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Article Navigation >  Nuclear Physics Review  > 2010  >  27(1): 82-86

WANG Shu-yan, CHE Ji-hong, LI Wen-jian, LIU Jing, CAO Yun-fei, BO Yong-heng, MA Xiao-qi, LIANG Jian-ping. Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+[J]. Nuclear Physics Review, 2010, 27(1): 82-86. doi: 10.11804/NuclPhysRev.27.01.082
Citation: WANG Shu-yan, CHE Ji-hong, LI Wen-jian, LIU Jing, CAO Yun-fei, BO Yong-heng, MA Xiao-qi, LIANG Jian-ping. Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+[J]. Nuclear Physics Review, 2010, 27(1): 82-86. doi: 10.11804/NuclPhysRev.27.01.082
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Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+

doi: 10.11804/NuclPhysRev.27.01.082

  • 1 Institute of Moden Physics, Chinese Academy of Sciences,Lanzhou 730000, China;2 Gansu Agriculture University, Lanzhou 730070, China

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2010-03-20
  • Mutagenic effect on avermectin B1a producing strains of ZJAV-A1 by ion beam of 12C6+ has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%， when ZJAVA1 was irradiated by ion beam of 50 Gy 12C6+. After the mutagenesis processing by ion beam of 12C6+ and the screening of orthomutation strains by using plating mediums and slant cultures，the mutant ZJAV-Y1203 was obtained with theavermectin B1a yield of 4588—4460 μg/ml. Compared with the original strain， the titer was improved 11.1%—14.7%.
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Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+

doi: 10.11804/NuclPhysRev.27.01.082
  • 1 Institute of Moden Physics, Chinese Academy of Sciences,Lanzhou 730000, China;2 Gansu Agriculture University, Lanzhou 730070, China
  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2010-03-20

Abstract: Mutagenic effect on avermectin B1a producing strains of ZJAV-A1 by ion beam of 12C6+ has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%， when ZJAVA1 was irradiated by ion beam of 50 Gy 12C6+. After the mutagenesis processing by ion beam of 12C6+ and the screening of orthomutation strains by using plating mediums and slant cultures，the mutant ZJAV-Y1203 was obtained with theavermectin B1a yield of 4588—4460 μg/ml. Compared with the original strain， the titer was improved 11.1%—14.7%.

WANG Shu-yan, CHE Ji-hong, LI Wen-jian, LIU Jing, CAO Yun-fei, BO Yong-heng, MA Xiao-qi, LIANG Jian-ping. Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+[J]. Nuclear Physics Review, 2010, 27(1): 82-86. doi: 10.11804/NuclPhysRev.27.01.082
Citation: WANG Shu-yan, CHE Ji-hong, LI Wen-jian, LIU Jing, CAO Yun-fei, BO Yong-heng, MA Xiao-qi, LIANG Jian-ping. Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+[J]. Nuclear Physics Review, 2010, 27(1): 82-86. doi: 10.11804/NuclPhysRev.27.01.082
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