Cytological Damage and Molecular Biology Effect of ¹²C⁶⁺ Heavy Ions on Allium fistulosum L.

Key words: 
• Allium fistulosum L. /
• ¹²C⁶⁺ heavy ion /
• chromosome aberration /
• Vitamin C /
• total watersoluble protein /
• RAPD analysis

Abstract: 
Abstract： The Allium fistulosum L. seeds were irradiated by 12C6+ heavy ions to the dosages of 30, 90, 180 Gy, the mutagenic effect in the aspect of the cell level and the agronomy was studied and the RAPD analysis was carried out. Comparision with the conclusion of M1 gengeration indicates that the Cytological damage, micronucleus and chromosomal aberration caused by radiation formed in the cells of Allium fistulosum L., and this kind of effect still existed in the M2 generation. There is a negative correlation between some of the growth indexes such as plant height, diameter of onion white and irradiation dosage to a certain extent, the growth indexes in the 30 Gy dosage exposure group are better than those in control group. The Allium fistulosum L. nutrients, including the total watersoluble protein and the Vitamin C content are the highest for the 30 Gy group and the lowest in 90 Gy group. Consistent with the M1 generation, the chromosomal aberrations, micronucleus and the DNA polymorphism rate by RAPD analysis are still positive correlations with the radiation dose in M2 generation respectively. However, the overall rates decline. The result indicated that the DNA variation induced by the high energy heavy ion exposure is repaired and eliminated to a certain extent in the M2 generation.