Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio

XU Hui-hui; SUI Li; LIU Xiao-dan; WANG Li; WANG Xiao; WANG Yu; XU Qin-zhi; ZHOU Ping-kun; #

doi:10.11804/NuclPhysRev.27.04.469

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Article Navigation > Nuclear Physics Review > 2010 > 27(4): 469-473

XU Hui-hui, SUI Li, LIU Xiao-dan, WANG Li, WANG Xiao, WANG Yu, XU Qin-zhi, ZHOU Ping-kun, #. Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio[J]. Nuclear Physics Review, 2010, 27(4): 469-473. doi: 10.11804/NuclPhysRev.27.04.469

Citation:

XU Hui-hui, SUI Li, LIU Xiao-dan, WANG Li, WANG Xiao, WANG Yu, XU Qin-zhi, ZHOU Ping-kun, #. Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio[J]. Nuclear Physics Review, 2010, 27(4): 469-473. doi: 10.11804/NuclPhysRev.27.04.469

Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio

doi: 10.11804/NuclPhysRev.27.04.469

1 Institute of Radiation Medicine of Military Academy of Medical Science, Beijing 100850 China;2 China Institute of Atomic Energy, Beijing 102413 China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2010-12-20

Abstract

Based on the molecular model of conformation changes of plasmid DNA， the DNA damages induced by γ rays， proton and ⁷Li ion were studied and compared. In order to detect the clustered DNA damage， the irradiated plasmid DNA was digested with E Coli formamidopyrimidine DNA glycosylase (Fpg) and AP endonuclease III (Endo III) which convert the damaged bases and AP sites into singlestrand breaks (SSB)， and then subjected to agarose gel electrophoresis. The results indicated that SSB， a certain level of clustered bases lesions and a tiny of doublestrand breaks (DSB)were induced by the observed 50—200 Gy of γ rays. The DNA breakage and clustered bases lesions induced by high LET proton and ⁷Li ion were much higher as compared with that induced by γ rays. Our data also showed that an obvious clustered DNA damage was induced by proton even at 10 Gy dose， and the effectiveness of DNA damage induction by proton was higher than that of ⁷Li ion.
- clustered DNA damage,
- heavy ion,
- proton,
- γ ray
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio

doi: 10.11804/NuclPhysRev.27.04.469

1 Institute of Radiation Medicine of Military Academy of Medical Science, Beijing 100850 China;2 China Institute of Atomic Energy, Beijing 102413 China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2010-12-20

Key words:

Abstract: Based on the molecular model of conformation changes of plasmid DNA， the DNA damages induced by γ rays， proton and ⁷Li ion were studied and compared. In order to detect the clustered DNA damage， the irradiated plasmid DNA was digested with E Coli formamidopyrimidine DNA glycosylase (Fpg) and AP endonuclease III (Endo III) which convert the damaged bases and AP sites into singlestrand breaks (SSB)， and then subjected to agarose gel electrophoresis. The results indicated that SSB， a certain level of clustered bases lesions and a tiny of doublestrand breaks (DSB)were induced by the observed 50—200 Gy of γ rays. The DNA breakage and clustered bases lesions induced by high LET proton and ⁷Li ion were much higher as compared with that induced by γ rays. Our data also showed that an obvious clustered DNA damage was induced by proton even at 10 Gy dose， and the effectiveness of DNA damage induction by proton was higher than that of ⁷Li ion.

Citation:

XU Hui-hui, SUI Li, LIU Xiao-dan, WANG Li, WANG Xiao, WANG Yu, XU Qin-zhi, ZHOU Ping-kun, #. Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio[J]. Nuclear Physics Review, 2010, 27(4): 469-473. doi: 10.11804/NuclPhysRev.27.04.469