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ZHANG Xin, ZHOU Xin, ZHANG Hong, #. A Novel Approach for Rapid Detection of Xray Irradiation Induced mtDNA D310 Mutation[J]. Nuclear Physics Review, 2011, 28(1): 118-121. doi: 10.11804/NuclPhysRev.28.01.118
Citation: ZHANG Xin, ZHOU Xin, ZHANG Hong, #. A Novel Approach for Rapid Detection of Xray Irradiation Induced mtDNA D310 Mutation[J]. Nuclear Physics Review, 2011, 28(1): 118-121. doi: 10.11804/NuclPhysRev.28.01.118
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A Novel Approach for Rapid Detection of Xray Irradiation Induced mtDNA D310 Mutation

doi: 10.11804/NuclPhysRev.28.01.118

  • 1 Institue of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2 Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China;3 Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China;4 Graduate University of Chinese Academy of Sciences, Beijing 100049, China

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2011-03-20
  • Human breast cancer cell line MCF7 was irradiated with 4 Gy Xray， collected at 0， 2， 4， 8， 16， 24， 48， 72, 144 h after irradiation， respectively. Whole genome DNA including mtDNA were extracted at each time point， and amplified by polymerase chain reaction(PCR). Then the PCR product was subjected to BSAXI digestion， all of digestion product then underwent a brief electrophoresis. Results showed D310 mutation can be induced by 4 Gy Xray irradiation and D310 mutation can overwhelm the normal phenotype 144 h after irradiation.
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A Novel Approach for Rapid Detection of Xray Irradiation Induced mtDNA D310 Mutation

doi: 10.11804/NuclPhysRev.28.01.118
  • 1 Institue of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2 Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China;3 Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China;4 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2011-03-20

Abstract:  Human breast cancer cell line MCF7 was irradiated with 4 Gy Xray， collected at 0， 2， 4， 8， 16， 24， 48， 72, 144 h after irradiation， respectively. Whole genome DNA including mtDNA were extracted at each time point， and amplified by polymerase chain reaction(PCR). Then the PCR product was subjected to BSAXI digestion， all of digestion product then underwent a brief electrophoresis. Results showed D310 mutation can be induced by 4 Gy Xray irradiation and D310 mutation can overwhelm the normal phenotype 144 h after irradiation.

ZHANG Xin, ZHOU Xin, ZHANG Hong, #. A Novel Approach for Rapid Detection of Xray Irradiation Induced mtDNA D310 Mutation[J]. Nuclear Physics Review, 2011, 28(1): 118-121. doi: 10.11804/NuclPhysRev.28.01.118
Citation: ZHANG Xin, ZHOU Xin, ZHANG Hong, #. A Novel Approach for Rapid Detection of Xray Irradiation Induced mtDNA D310 Mutation[J]. Nuclear Physics Review, 2011, 28(1): 118-121. doi: 10.11804/NuclPhysRev.28.01.118
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