Optimization of Culture Medium for Heavy-ion Irradiation Bread Yeast Design

MA Liang; WANG Jufang; LU Dong; LI Wenjian; XIAO Guoqing

doi:10.11804/NuclPhysRev.30.02.201

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MA Liang, WANG Jufang, LU Dong, LI Wenjian, XIAO Guoqing. Optimization of Culture Medium for Heavy-ion Irradiation Bread Yeast Design[J]. Nuclear Physics Review, 2013, 30(2): 201-205. doi: 10.11804/NuclPhysRev.30.02.201

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MA Liang, WANG Jufang, LU Dong, LI Wenjian, XIAO Guoqing. Optimization of Culture Medium for Heavy-ion Irradiation Bread Yeast Design[J]. Nuclear Physics Review, 2013, 30(2): 201-205. doi: 10.11804/NuclPhysRev.30.02.201

Optimization of Culture Medium for Heavy-ion Irradiation Bread Yeast Design

doi: 10.11804/NuclPhysRev.30.02.201

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2013-06-20

Abstract

A mutant bread yeast strain with high protein content of 55% was gained by use of ¹²C⁶⁺ ions. The MINITAB 16.0 software, Plackett-Burman experimental design and response surface methodology were applied to optimize the culture medium for the irradiated yeast. The most important three factors which influenced the culture results were identified as glucose, magnesium sulphate and yeast extract. The path of the steepest ascent was undertaken to approach the optimal region of the three significant factors. Box-Behnken design and response surface methodology were used for the regression analysis. Finally, the optimal fermentation conditions were identified as glucose 11.03 g/L, yeast extract 6.53 g/L and magnesium sulphate 5.59 g/L by the regression analysis. It was found that the biomass of the bread yeasts reached 4.84 g/L and increased by 15% compared to original conditions.
- heavy ion irradiation,
- bread yeast,
- response surface methodology,
- Plackett-Burman
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Optimization of Culture Medium for Heavy-ion Irradiation Bread Yeast Design

doi: 10.11804/NuclPhysRev.30.02.201

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2013-06-20

Key words:

Abstract: A mutant bread yeast strain with high protein content of 55% was gained by use of ¹²C⁶⁺ ions. The MINITAB 16.0 software, Plackett-Burman experimental design and response surface methodology were applied to optimize the culture medium for the irradiated yeast. The most important three factors which influenced the culture results were identified as glucose, magnesium sulphate and yeast extract. The path of the steepest ascent was undertaken to approach the optimal region of the three significant factors. Box-Behnken design and response surface methodology were used for the regression analysis. Finally, the optimal fermentation conditions were identified as glucose 11.03 g/L, yeast extract 6.53 g/L and magnesium sulphate 5.59 g/L by the regression analysis. It was found that the biomass of the bread yeasts reached 4.84 g/L and increased by 15% compared to original conditions.

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