Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901

MIAO Guoying; ZHANG Hong; LU Qiming; LI Hongyan; DI Cuixia; GUO Yixiao

doi:10.11804/NuclPhysRev.32.01.110

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Article Navigation > Nuclear Physics Review > 2015 > 32(1): 110-114

MIAO Guoying, ZHANG Hong, LU Qiming, LI Hongyan, DI Cuixia, GUO Yixiao. Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901[J]. Nuclear Physics Review, 2015, 32(1): 110-114. doi: 10.11804/NuclPhysRev.32.01.110

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MIAO Guoying, ZHANG Hong, LU Qiming, LI Hongyan, DI Cuixia, GUO Yixiao. Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901[J]. Nuclear Physics Review, 2015, 32(1): 110-114. doi: 10.11804/NuclPhysRev.32.01.110

Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901

doi: 10.11804/NuclPhysRev.32.01.110

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy ofSciences, Lanzhou 730000, China;
Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China;
University of Chinese Academy of Sciences, Beijing 100049, China;

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2015-03-20

Abstract

Human gastric cancer cell SGC7901 were irradiated with high linear energy transfer (LET) carbon ion. Apoptotic cells after irradiation were analyzed by flow cytometry and expression of MSH2 genes in the irradiated cells was detected by western blot and RT-PCR assay. Compared with the control group, we found that the number of G2/M (33.26±0.08) or apoptosis (24.16±0.64) of SGC7901 cells reached a maximum after irradiation at 72 h in a dose dependent manner. And heavy ion irradiation efficiently up-regulated the expression of MSH2 gene at 4.0 Gy after being irradiated 6 h. These results imply that heavy ion beam could induce cell apoptosis and cell cycle arrest in time-dependent manners. Furthermore, expression of MSH2 genes activated by carbon ion irradiation suggests that DNA mismatch repair gene MSH2 might be involved in DNA repair pathways.
- cell apoptosis,
- heavy ion beam,
- MSH2,
- gene expression
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901

doi: 10.11804/NuclPhysRev.32.01.110

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;

Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy ofSciences, Lanzhou 730000, China;

Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China;

University of Chinese Academy of Sciences, Beijing 100049, China;

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2015-03-20

Key words:

Abstract: Human gastric cancer cell SGC7901 were irradiated with high linear energy transfer (LET) carbon ion. Apoptotic cells after irradiation were analyzed by flow cytometry and expression of MSH2 genes in the irradiated cells was detected by western blot and RT-PCR assay. Compared with the control group, we found that the number of G2/M (33.26±0.08) or apoptosis (24.16±0.64) of SGC7901 cells reached a maximum after irradiation at 72 h in a dose dependent manner. And heavy ion irradiation efficiently up-regulated the expression of MSH2 gene at 4.0 Gy after being irradiated 6 h. These results imply that heavy ion beam could induce cell apoptosis and cell cycle arrest in time-dependent manners. Furthermore, expression of MSH2 genes activated by carbon ion irradiation suggests that DNA mismatch repair gene MSH2 might be involved in DNA repair pathways.

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