MA Binbo, WANG Zhuanzi, WEI Wei, DANG Bingrong, LI Wenjian. Effects of Carboxymethy-β-1,3-glucan on Human Hepatoma HepG2 Cells Radiosensitivity[J]. Nuclear Physics Review, 2017, 34(4): 797-802. doi: 10.11804/NuclPhysRev.34.04.797
Citation:
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MA Binbo, WANG Zhuanzi, WEI Wei, DANG Bingrong, LI Wenjian. Effects of Carboxymethy-β-1,3-glucan on Human Hepatoma HepG2 Cells Radiosensitivity[J]. Nuclear Physics Review, 2017, 34(4): 797-802. doi: 10.11804/NuclPhysRev.34.04.797
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Effects of Carboxymethy-β-1,3-glucan on Human Hepatoma HepG2 Cells Radiosensitivity
- 1.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
- 2.
Lanzhou University, Lanzhou 730000, China
Funds:
National Natural Science Foundation of China(11575259)
- Received Date: 2016-12-28
- Rev Recd Date:
2017-01-10
- Publish Date:
2017-12-20
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Abstract
This study aims to investigate the effect of carboxymethy-β-1, 3-glucan (CMG) on the sensitivity of human hepatoma HepG2 cells to X-rays or 12C6+ ions irradiation. First, the inhibitory effect of CMG on the growth of HepG2 cells was detected by CCK-8 assay, and the half maximal inhibitory concentration (IC50) was 120.6 μg/mL. HepG2 cells were pretreated with CMG at a concentration of 0.1×IC50 for 24 h and then irradiated with 2 Gy X-ray or 12C6+ ion beams (CMG + irradiation group). CMG untreated group was directly irradiated by 2 Gy X-rays or 12C6+ ions beam (irradiation group). The clone survival, DNA damage, cell apoptosis, cell cycle distribution, and intracellular reactive oxygen species (ROS) levels in irradiation group and CMG + irradiation group were comparatively analyzed. The results showed that the clone survival rate was lower, DNA damage and cycle arrest were more serious, and the rate of apoptosis and intracellular ROS levels were higher in 12C6+ ions irradiation group than those in the same dose of X-rays irradiation group. Compared with X-rays or 12C6+ ions irradiation group, the clone survival rate of CMG + irradiation group was significantly decreased, and the apoptosis rate significantly increased with the prolongation of CMG treatment post-irradiation; CMG maintained intracellular ROS at a higher level after irradiation, CMG also significantly aggravated radiation-induced DNA damage and cycle arrest. These results indicated that HepG2 cells were more sensitive to 12C6+ ions radiation than those at the same dose of X-rays. CMG increased the sensitivity of HepG2 cells to X-rays or 12C6+ ions irradiation by increasing intracellular ROS level, exacerbating radiation-induced DNA damage and promoting radiation-induced apoptosis in irradiated HepG2 cells.
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Proportional views
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