Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells

MING Xingyao; LIU Bingtao; MA Xiaohui; ZHANG Yang; CHEN Weiqiang; JIN Xiaodong

doi:10.11804/NuclPhysRev.36.01.104

The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.

Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells

1. Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;

2. Key Laboratory of Dunhuang Medicine Ministry of Education, Lanzhou 730000, China;

3. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;

4. School of Nuclear Science and Technology, University of Chinese Academy of Sciences, Beijing 100049, China

Funds: National Natural Science Foundation of China (U1730133); Natural Science Foundation of Gansu Province (17JRSRA310)

Received Date: 2018-12-14

Rev Recd Date: 2019-02-02

Publish Date: 2019-03-20

Key words:

Abstract: The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.

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Reference (19)

[1]	BRAY F, FERLAY J, SOERJOMATARAM I, et al. CA Cancer J Clin, 2018, 68:394.
[2]	AUPERIN A, C LE PECHOUX, ROLLAND E, et al. J Clin Oncol, 2010, 28:2181.
[3]	OLEINICK N L, BISWAS T, PATEL R, et al. Radiother Oncol 2016, 121:335.
[4]	KAMADA T, TSUJⅡ H, BLAKELY E A, et al. Lancet Oncol, 2015, 16:e93.
[5]	PAN J, ZHANG Q, LI K, et al. Cancer Prev Res (Phila), 2013, 6:530.
[6]	PAN J, LEE Y, WANG Y, et al. Mol Nutr Food Res, 2016, 60:1383.
[7]	HE Z, SUBRAMANIAM D, RAMALINGAM S, et al. Am J Physiol Gastrointest Liver Physiol, 2011, 301:G929.
[8]	PONNURANGAM S, MAMMEN J M, RAMALINGAM S, et al. Mol Cancer Ther, 2012, 11:963.
[9]	HU J, CHEN L J, LIU L, et al. Exp Mol Med, 2008, 40:617.
[10]	HE Z, SUBRAMANIAM D, ZHANG Z, et al. Curr Colorectal Cancer Rep, 2013, 9:358.
[11]	OKAYASU R. Int J Cancer, 2010, 130:991.
[12]	WANG X, BEITLER J J, HUANG W, et al. Clin Cancer Res, 2018, 24:858.
[13]	JIN X D, GONG L, GUO C L, et al. Radiat Environ Biophys, 2008, 47:399.
[14]	JIN X, LI Q, WU Q, et al. J Radiat Res,2011, 52:335.
[15]	DU M, WANG J, CHEN H, et al. Oncol Rep, 2018(12):1497.
[16]	RUAN W, LIM H H, SURANA U, et al. Front Cell Dev Biol, 2018, 6:177.
[17]	SHENG Y L, XU J H, SHI C H, et al. J Ethnopharmacol, 2014, 155:1568.
[18]	WANG X, DUAN X, YANG G, et al. PLoS One 2011, 6:e18490.
[19]	ZHANG Q, LI J, ZHANG W, et al. Regul Toxicol Pharmacol, 2015, 71:428.

Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells

doi: 10.11804/NuclPhysRev.36.01.104

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