Effects of Carbon Ion Irradiation on Survival Rate,Catalase and Peroxidase Activity of Alfalfa M1 under Low Temperature Stress

Key words: 
• ¹²C⁶⁺ ion irradiation /
• low temperature stress /
• survival rate /
• catalase activity /

Abstract: In this study, three kinds of alfalfa including Zhonglan 1, BC-04-477 and Ta Cheng were treated with different doses of ¹²C⁶⁺ (75 keV) heavy ion radiation, and then the influence of survival rate, catalase(CAT) and peroxidase(POD) activity of M1 with low temperature stress were tested. The results showed that under the condition of 400 Gy radiation dose , the survival rate and CAT activity of Zhonglan 1 under low temperature stress have increased by 33.3%, 56.3% respectively compared with those of the control group, while there was no difference in POD activity between those two groups. The survival rate, CAT and POD activity of BC-04-477 treated with low temperature have been improved by 33.3%, 69.2%, 5.1% respectively compared with those of the control group when the radiation dose was 400 Gy. Compared with those of the control group, the survival rate, CAT and POD activity of Ta Cheng under low temperature stress have been improved by 25%, 26%,22.8% respectively when the radiation dose was 800 Gy. These results indicate that the viability and the cold resistance ability of Zhong Lan 1, BC-04-477 and Ta Cheng can be improved by ¹²C⁶⁺ radiation.